USC School of Medicine

Articles using vectors from the PPN Virus Vector Lab

Grillo CA, Tamashiro KL, Piroli GG, Melhorn S, Gass JT, Newsom RJ, Reznikov LR, Smith A, Wilson SP, Sakai RR, Reagan LP (2007) Lentivirus-mediated downregulation of hypothalamic insulin receptor expression. Physiol Behav.

Jones TL, Sweitzer SM, Peters MC, Wilson SP, Yeomans DC (2005) GABAB receptors on central terminals of C-afferents mediate intersegmental Adelta-afferent evoked hypoalgesia. Eur J Pain 9: 233-242. Jones TL, Sweitzer SM, Wilson SP, Yeomans DC (2003) Afferent fiber-selective shift in opiate potency following targeted opioid receptor knockdown. Pain 106: 365-371.

Kang W, Wilson MA, Wilson SP (2000) Overexpression of proenkephalin in the amygdala potentiates the anxiolytic effects of benzodiazepines. Neuropsychopharm 22: 77-88. Primeaux SD, Wilson SP, Bray GA, York DA, Wilson MA (2006a) Overexpression of neuropeptide Y in the central nucleus of the amygdala decreases ethanol self-administration in "anxious" rats. Alcohol Clin Exp Res 30: 791-801.

Primeaux SD, Wilson SP, McDonald AJ, Mascagni F, Wilson MA (2006b) The role of delta opioid receptors in the anxiolytic actions of benzodiazepines. Pharmacol Biochem Behav 85: 545-554.

Tzabazis AZ, Pirc G, Votta-Velis E, Wilson SP, Laurito CE, Yeomans DC (2007) Antihyperalgesic effect of a recombinant herpes virus encoding antisense for calcitonin gene-related peptide. Anesthesiology 106: 1196-1203.

Wilson SP, Yeomans DC, Lu Y, Bender MA, Glorioso JC (1999) Antihyperalgesic effects of infection with a proenkephalin-encoding herpes virus. Proc Natl Acad Sci U S A 96: 3211-3216.

Consultation
Viral Vectors
Design
Issues include:
- Choice of vector
- Choice of genetic elements and market genes
- Source of cDNA or miRNA sequences
- Appropriate control virus
Construction
Isolation of Recombinants (herpes only)
Production
DeliveryThe minimum times required for vector production are 3 months for lentivirus and 6 months for herpesvirus. These lead times assume that 20 µg of a plasmid containing the cDNA or miRNA is on hand.
Molecular Cloning