building 3

Collection of the plasmids

We are pleased to stock AAV- and LV-optogenetic constructs from Dr. Karl Deisseroth at Stanford University.  For first time order of these vectors you will have to fill an MTA form with Dr. Deisseroth. To obtain MTA, please contact Dr. Karl Deisseroth and Charu Ramakrishnan. Please upload a copy of your MTA when placing your online order.
Contact email of Charu Ramakrishnan: charur@stanford.edu
Contact email of Dr. Karl Deisseroth: deissero@stanford.edu

AAV constructs

  • AAV-EF1a-DIO-EYFP
  • AAV-EF1a-DIO-mCherry
  • AAV-EF1a-DIO-hCHR2(H134R)-mCherry
  • AAV-EF1a-DIO-hCHR2(H134R)-EYFP
  • AAV-EF1a-DIO-eNpHR 3.0-EYFP
  • AAV-EF1a-DIO-chETA-EYFP
  • AAV-CaMKIIa- EYFP
  • AAV-CaMKIIa- mCherry
  • AAV-CaMKIIa- hCHR2(H134R)-EYFP
  • AAV-CaMKIIa- hCHR2(H134R)-mCherry
  • AAV-CaMKIIa- eNpHR 3.0-EYFP
  • AAV-hSyn- hCHR2(H134R)-EYFP
  • AAV-hSyn- hCHR2(H134R)-mCherry
  • AAV-hSyn- eNpHR 3.0-EYFP
  • AAV-CaMKIIa- C1V1 (t/t)-TS- EYFP
  • AAV-CaMKIIa- C1V1 (t/t)-TS- mCherry
  • AAV-EF1a-DIO- C1V1 (t/t)-TS- EYFP
  • AAV-EF1a-DIO- C1V1 (t/t)-TS- mCherry
  • AAV-CaMKIIa- hCHR2(C128S/D156A)-EYFP
  • AAV-CaMKIIa- hCHR2(C128S/D156A)-mCherry
  • AAV-EF1a-DIO- hCHR2(C128S/D156A)-EYFP
  • AAV-EF1a-DIO- hCHR2(C128S/D156A)-mCherry
  • AAV- CaMKIIa - hCHR2(et/tc)-EYFP
  • AAV- CaMKIIa - hCHR2(et/tc)-mCherry
  • AAV-CaMKIIa- hCHR2(E123A)-EYFP
  • AAV-CaMKIIa- hCHR2(E123A)-mCherry
  • AAV-EF1a-DIO- hCHR2(et/tc)-EYFP
  • AAV-EF1a-DIO- hCHR2(et/tc)-mCherry
  • AAV-EF1a-DIO- hCHR2(E123A)-EYFP
  • AAV-EF1a-DIO- hCHR2(E123A)-mCherry
  • AAV-EF1a-DIO- eNpHR 3.0- mCherry
  • AAV- CaMKIIa- eNpHR 3.0- mCherry
  • AAV- hSyn- eNpHR 3.0- mCherry
  • AAV- hSyn- hCHR2(E123A)-EYFP
  • AAV- hSyn- EYFP
  • AAV-EF1a-mCherry-IRES-WGA-Cre
  • AAV-GFAP-DIO-hCHR2(H134R)-EYFP
  • AAV-GFAP-DIO-hCHR2(H134R)-mCherry
  • AAV- CaMKIIa- eArch3.0- EYFP
  • AAV-EF1a-DIO- eArch3.0- EYFP
  • AAV- CaMKIIa- eArchT3.0- EYFP
  • AAV- hSyn- mCherry
  • AAV-EF1a-DIO- hCHR2(T159C)- EYFP
  • AAV-EF1a-DIO- hCHR2(T159C)- mCherry
  • AAV- hSyn- eArch3.0- EYFP
  • AAV- hThy-1- eNpHR 3.0- EYFP
  • AAV- CaMKIIa- C1V1 (E162T)- p2A- EYFP
  • AAV-CaMKIIa- hCHR2(T159C)- p2A- EYFP
  • AAV-CaMKIIa- hCHR2(T159C)- p2A- mCherry
  • AAV-CaMKIIa- hCHR2(et/tc)- p2A- EYFP
  • AAV-CaMKIIa- hCHR2(et/tc)- p2A- mCherry
  • AAV-CaMKIIa- C1V1 (E162T)- TS-p2A- EYFP
  • AAV-CaMKIIa- C1V1 (E162T)- TS-p2A- mCherry
  • AAV-CaMKIIa- C1V1 (t/t)- TS-p2A- EYFP
  • AAV-CaMKIIa- C1V1 (t/t)- TS-p2A- mCherry
  • AAV-EF1a-DIO- hCHR2(et/tc)- p2A- EYFP
  • AAV-EF1a-DIO- hCHR2(et/tc)- p2A- mCherry
  • AAV-EF1a-DIO- C1V1(E162T)- TS- p2A- EYFP
  • AAV-EF1a-DIO- C1V1(E162T)- TS- p2A- mCherry
  • AAV-EF1a-DIO- C1V1(t/t)- TS- p2A- EYFP
  • AAV-EF1a-DIO- C1V1(t/t)- TS- p2A- mCherry

Lentiviral constructs

  • pLenti- CaMKIIa- eArchT3.0- EYFP
  • pLenti- CaMKIIa- eArch3.0- EYFP
  • pLenti- CaMKIIa- eMac3.0- EYFP
  • pLenti- CaMKIIa- C1V1(E122T)-TS- EYFP
  • pLenti- CaMKIIa- C1V1(E162T)-TS- EYFP
  • pLenti- CaMKIIa- C1V1(E122T/E162T)-TS- EYFP
  • pLenti- CaMKIIa- hCHR2(C128S/D156A)- EYFP
  • pLenti- CaMKIIa- hCHR2(T159C)- EYFP-WPRE
  • pLenti- CaMKIIa- hCHR2(E123T/T159C)- EYFP-WPRE
  • pLenti- EF1a - hCHR2- EYFP-WPRE (a.k.a. pLECYT)
  • pLenti- CaMKIIa- hCHR2-mCherry-WPRE
  • pLenti- CaMKIIa- hCHR2- EYFP -WPRE
  • pLenti- CaMKIIa- hCHR2(H134R)- EYFP -WPRE
  • pLenti- hSyn - hCHR2(H134R)- EYFP –WPRE
  • pLenti- CaMKIIa- eNpHR 3.0- EYFP
  • pLenti- hSyn- eNpHR 3.0- EYFP

We are pleased to stock LV-optogenetic constructs from Dr. Ed Boyden at MIT. No MTA required for ordering these plasmids.

Lentiviral constructs

  • FCK-Halo-GFP
  • FCK-ChR2-GFP
  • FCK-Arch-GFP
  • FCK-Mac-GFP
  • FCK-ArchT-GFP

Please note that the FCK and the CaMKIIa promoters are the same.

We also offer the following plasmids developed in our laboratory.

  • pLenti-mPGK-LacZ
  • pLenti-mPGK-EGFP
  • pLenti-mPGK-mCherry
  • pLenti-mPGK-Luc-IRES-EGFP
  • pLenti-mPGK-AcGFP
  • pLenti-hCMV-EGFP
  • pLenti-hCMV-mCherry
  • pLenti-mPGK-LacZ
  • pLenti-hSyn- EGFP
  • pLenti-hSyn- mCherry
  • pLenti-hSyn- mCherry-IRES-EGFP
  • pLenti-hSyn- Luc-PGK-GFP
  • pLenti-FCK- EGFP
  • pLenti-hGFAP- EGFP